FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n01_B2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n01_B2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26551795
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC327300.1232685021860104TruSeq Adapter, Index 6 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305400.11502047225055784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG65500.025.89114243
CTCGTAT66200.025.7534542
AGTCACG73500.025.18738228
ACGTCTG78150.024.14933215
CGTATGC72700.023.26544
CGTCTGA81650.023.11410916
GTCACGC85550.021.9551829
ACGCCAA89500.020.78588732
TATCTCG86200.020.76434339
CGCCAAT90700.020.29110333
TCGGAAG97100.020.0192433
ATCGGAA99500.019.468532
CACGCCA96300.019.4342431
CACGTCT99350.019.22259314
CAGTCAC100600.018.93895327
GTATGCC92650.018.64436145
TCACGCC103250.018.3875130
CACACGT108900.017.7640812
ACACGTC111300.017.52250113
GCCAATA106650.017.4694134
Produced by FastQC (version 0.11.5)