FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n01_B2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n01_B2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22673758
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC237530.10475987262455566TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46450.025.74547243
CTCGTAT46800.025.07170742
ACGTCTG50300.024.3722415
CGTCTGA52300.023.44022216
CGTATGC57300.021.14558644
GTCACAC62250.019.72968729
CACGTCT64450.019.4401314
TCGGAAG65850.019.3724353
ATCGGAA68650.018.5167522
CAGTCAC68000.018.06136727
CACACGT73550.017.4630612
AGTCACA71550.016.8508628
AGTGATC71850.016.25462736
GTATGCC77450.016.05128345
ACACGTC82250.015.80734213
CACAGTG78600.015.28586533
CGGAAGA91900.014.3707544
ACACAGT84800.014.324615532
CAGTGAT84850.014.26812635
CCAGTCA86600.014.13018626
Produced by FastQC (version 0.11.5)