FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n01_B2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n01_B2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26628213
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG50450.018.45884115
CGTCTGA51600.017.56793216
CTCGTAT42850.016.6616842
TCGTATG43350.016.52145843
TCGGAAG61900.015.0472863
CGTATGC45300.015.01478544
ATCGGAA64050.014.4016822
AGTCACT67500.013.829628
ACTGACC66800.013.77330732
CACGTCT71150.013.15175514
CACACGT73150.012.63842112
ACACGTC76800.012.35994413
AATCTCG70000.012.15818139
GATCGGA80550.011.6209471
GTCTGAA89450.010.51147617
GTCACTG89500.010.50554529
GTATGCC67800.010.49706245
CAGTCAC91850.010.43267627
CACTGAC92400.010.27320131
TCTCGTA75050.09.96250141
Produced by FastQC (version 0.11.5)