FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n01_B2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n01_B2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22437467
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT40300.024.70045542
TCGTATG40550.024.0483243
ACTTAGG49900.023.93762632
ACGTCTG52900.022.7919115
CGTCTGA55650.021.74646816
CGTATGC45250.021.25186344
TTAGGCA57650.020.45238334
TCACTTA58650.020.21222730
CTTAGGC59450.020.09570733
TCGGAAG65550.018.9462033
AGTCACT64850.018.38389428
ATCGGAA68800.017.7242012
GTCACTT67450.017.575229
CACGTCT71800.016.91768514
CACACGT77650.015.81695112
CAGTCAC79450.015.54354327
TAGGCAT78650.015.27812335
TCTCGTA68200.015.22178341
ACACGTC84000.014.91583313
GATCGGA88950.013.9893931
Produced by FastQC (version 0.11.5)