FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n01_B2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n01_B2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26388697
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC569720.21589546463775758TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG85500.032.53029643
CTCGTAT87350.032.30541642
CGTATGC89750.030.66365444
ACGTCTG123300.026.83621815
TATCTCG114200.026.61428339
CGTCTGA125950.026.25372116
CACCGAT123500.026.2099131
ACCGATG124700.025.95867532
GATGTAT123350.025.94164835
GTATCTC135100.023.4762938
TCTCGTA121250.023.4203941
ATCGGAA146950.022.9961972
CACGTCT145500.022.9580614
CAGTCAC144700.022.68080527
TCGGAAG149300.022.6191623
CACACGT150850.022.35259812
GTATGCC127050.022.1398645
AGTCACC149800.022.07380528
TGTATCT146700.021.90708537
ACACGTC164350.020.68075813
Produced by FastQC (version 0.11.5)