FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l06_n01_B2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l06_n01_B2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23149509
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC421130.18191746529051653TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG79300.032.0536615
TCGTATG72050.031.44357943
CGTCTGA81150.031.23976716
CTCGTAT71600.030.0371242
CGTATGC77800.028.7723144
TCGGAAG94800.027.2221953
CACGTCT96850.026.3614114
ATCGGAA98250.026.1060032
CACACGT99900.025.87181912
TCACGAT99000.024.7759735
CAGTCAC105000.024.05818627
GTCACAT104900.023.93101529
AGTCACA107900.023.47412128
ACACGTC114500.022.61217113
ATCACGA109500.022.56401334
GATCGGA117250.021.9743061
TCTCGTA99950.021.67397941
CATCACG119050.021.166633
CGGAAGA123650.021.1072644
AGCACAC126550.021.01008210
Produced by FastQC (version 0.11.5)