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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-04-24, 18:04 based on data in: /beegfs/mk5636/logs/html/CCRMBACXX/5

        General Statistics

        Showing 20/20 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        CCRMBACXX_l05_n01_E2S1
        67.1%
        52%
        24.5
        CCRMBACXX_l05_n01_E2S2
        68.8%
        53%
        25.7
        CCRMBACXX_l05_n01_E2S3
        68.3%
        52%
        26.0
        CCRMBACXX_l05_n01_E2S4
        67.9%
        52%
        27.0
        CCRMBACXX_l05_n01_E2S5
        67.4%
        53%
        24.2
        CCRMBACXX_l05_n01_E2S6
        66.7%
        52%
        24.0
        CCRMBACXX_l05_n01_E2S7
        59.4%
        51%
        12.4
        CCRMBACXX_l05_n01_E2S8
        67.1%
        53%
        23.5
        CCRMBACXX_l05_n01_E2S9
        66.4%
        53%
        22.3
        CCRMBACXX_l05_n01_undetermined
        89.7%
        45%
        17.8
        CCRMBACXX_l05_n02_E2S1
        69.1%
        55%
        24.5
        CCRMBACXX_l05_n02_E2S2
        70.4%
        55%
        25.7
        CCRMBACXX_l05_n02_E2S3
        70.3%
        54%
        26.0
        CCRMBACXX_l05_n02_E2S4
        70.4%
        54%
        27.0
        CCRMBACXX_l05_n02_E2S5
        69.7%
        55%
        24.2
        CCRMBACXX_l05_n02_E2S6
        67.9%
        54%
        24.0
        CCRMBACXX_l05_n02_E2S7
        61.4%
        53%
        12.4
        CCRMBACXX_l05_n02_E2S8
        69.1%
        55%
        23.5
        CCRMBACXX_l05_n02_E2S9
        68.2%
        54%
        22.3
        CCRMBACXX_l05_n02_undetermined
        89.7%
        46%
        17.8

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        5.0
        238,016,400
        227,315,864
        7.8
        7.9

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        NNNNNN
        140140.0
        0.8
        CGGCCC
        75061.0
        0.4
        TAGGCA
        70543.0
        0.4
        CAGCCC
        62084.0
        0.3
        CCCCCC
        58778.0
        0.3
        CCGCCC
        58677.0
        0.3
        GATGTA
        55939.0
        0.3
        CGGCCA
        55218.0
        0.3
        GACCAA
        51609.0
        0.3
        CGCCCC
        50487.0
        0.3
        GCCCAA
        47421.0
        0.3
        CCGGTC
        45852.0
        0.3
        CACCCC
        45359.0
        0.3
        GACAAC
        44368.0
        0.2
        GACCAC
        44062.0
        0.2
        ATACGA
        43858.0
        0.2
        ACTGAA
        43186.0
        0.2
        ATCAGA
        42797.0
        0.2
        CAGCAC
        42353.0
        0.2
        TGCCCC
        42286.0
        0.2

        Lane 5 Demultiplexing Report

        Lane 5 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 10/10 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        17,759,218
        7.8
        E2S1
        24,523,154
        10.8
        E2S2
        25,675,051
        11.3
        E2S3
        25,972,064
        11.4
        E2S4
        27,000,950
        11.9
        E2S5
        24,152,919
        10.6
        E2S6
        23,978,070
        10.5
        E2S7
        12,437,343
        5.5
        E2S8
        23,505,833
        10.3
        E2S9
        22,311,262
        9.8

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (51bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        20 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.