FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17759218
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT8900.023.5264052
GAGTGTA6300.018.57356628
TAGATCT6150.017.56136533
AGTGTAG6400.017.22859429
GTAGATC6350.017.00959232
TCTCGGT6200.016.32792537
GTGTAGA7150.016.05079830
TCCCTAC6100.014.0149669
TGTAGAT8400.013.12643731
TACACGA5350.013.03543213
GTGTAGG8650.012.74375617
GTACTCG5350.012.2021354
GTAGAAC3701.8189894E-1212.16092311
GTAGGGA9100.012.11401519
TTCCCTA7450.012.0789338
TCGTGTA9450.011.90291215
TAGGGAA9550.011.54329620
AGTACTC5950.011.3508013
GATCTCG10350.011.08523535
TGTAGGG10000.011.02353718
Produced by FastQC (version 0.11.5)