FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n02_E2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n02_E2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22311262
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT20250.013.4536432
GTACTCG51850.013.06813054
AGTACTC57650.012.0668213
TACTCGA64050.010.5084355
GCGTGAC29400.09.9562551
GGAAAAT88700.09.4685881
GTAGAAC28150.09.27054911
GTGACTC32100.08.9771543
ACATGCG28850.08.812116
TGCGAGT27300.08.73481113
TCCGTTA43300.08.728888519
ACTCGAG82150.08.7089716
CTCGAAT20300.08.6516431
CTCTATA44450.08.55297945
CGCGATA10600.08.49166829
GCGATTT56250.08.4862011
CGTTAAC45950.08.42208221
GCGGAAA50150.08.3510681
ACTTTCG21300.08.34420719
AAAATCC72250.08.2573713
Produced by FastQC (version 0.11.5)