FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n02_E2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n02_E2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23505833
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG51600.013.3933114
TACTCGA55500.012.1679755
AGTACTC60800.011.8490023
CGTGACT21400.011.6784762
GTAGAAC35750.011.39021111
TCAGGTA45200.010.1035497
GCGGAAA51250.09.9732071
CACTCGT46050.09.67262835
CTCTATA45300.09.633821545
GGAAAAT105550.09.4290281
TAGAACA46050.09.18413212
GAATCGA33600.09.1786874
AATCGAA37600.09.1599615
GCGTGAC32150.09.1046921
TCACTCG48350.09.07320934
GGTAGAA47600.08.97999110
CGGAAAC55450.08.7705972
ACTCGTG50100.08.756000536
TTACGAC24400.08.39261629
CTCGAAT20400.08.2781641
Produced by FastQC (version 0.11.5)