FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n02_E2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n02_E2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12437343
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC20200.014.5899511
GTACTCG21750.011.9024014
GGTAGAA26200.011.50629810
TAGAACA27250.011.14500212
TCAGGTA28900.011.1315687
TACTCGA26800.09.8271965
CAGGTAG30350.09.7102588
AGTACTC28800.09.6150293
AGATCAG47100.09.1764844
AGGTAGA34600.08.8429049
AATCCAG31100.08.2513115
GGAAAAT49900.08.0320031
CGGCTAT13300.07.9570231
AGAACGG71850.07.92741733
CACGTAA15150.07.86986716
CGTGTTA14350.07.8435764
AACGGCC52150.07.76956565
CGACGAT45350.07.6958891
AGAGATC56600.07.6774252
TGAGAGT41100.07.66337520
Produced by FastQC (version 0.11.5)