FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n02_E2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n02_E2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23978070
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC35700.012.47762411
GTACTCG44250.011.3445594
CGTGACT24600.010.7999962
CACTCGT62500.010.11438535
ACTCGTG63550.010.01798636
AGTACTC55500.09.9382913
TACTCGA51400.09.8100425
GCGGAAA70550.09.7980131
TCAGGTA48400.09.6680797
GGTAGAA46950.09.63153310
GCGTGAC31750.09.50293351
CTCTATA66150.09.45401745
TAGAACA47650.09.44247212
TCACTCG67450.09.27230734
CAGGTAG50150.09.0615548
GGAAAAT93800.08.8336781
CGGAAAC76350.08.522472
CTAGTTG77950.08.456099513
GCTAGTT80000.08.32377512
AATCGAA41950.08.2100445
Produced by FastQC (version 0.11.5)