FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n02_E2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n02_E2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24152919
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG55900.012.7656874
CGTGACT25500.012.6260442
TACTCGA59750.011.9428815
AGTACTC62800.011.6866913
GTAGAAC33800.011.24874211
GCGTGAC32850.010.3499281
CTCTATA64750.09.55414445
GTGACTC38300.09.1697913
AAAATCC69850.09.0567753
GCGGAAA70400.09.051271
TCAGGTA47000.09.0464857
CACTCGT66000.09.03259435
TAGAACA43150.09.019381512
ACTCGTG67950.08.83955936
TCACTCG68850.08.72426234
GGAAAAT96950.08.6627691
ACTCGAG88600.08.6591866
GGTAGAA45200.08.61072710
CGGCTAT30250.08.5598911
CTCGAAT18050.08.482571
Produced by FastQC (version 0.11.5)