FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n02_E2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n02_E2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27000950
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG60100.013.1095614
CGTGACT25350.012.6119752
TACTCGA67200.011.6571965
AGTACTC71450.011.1224393
GTAGAAC33600.010.71296411
GCGTGAC34050.010.5803221
CACTCGT68200.010.29158435
CTCTATA70000.09.93027345
GCGGAAA76600.09.6708161
TCACTCG71900.09.5432434
ACTCGTG71900.09.48027436
ACTCGAG104300.08.4772796
GTGACTC41900.08.2743583
GCTAGTT87100.08.26503412
GGAAAAT109300.08.1165611
CGGAAAC88350.08.1038852
CTCGTGA89800.08.09150637
CTAGTTG88700.07.989135313
TAGAACA45950.07.980221312
GCTCTAT88250.07.978667744
Produced by FastQC (version 0.11.5)