FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n02_E2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n02_E2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25972064
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG58800.012.7486684
CGTGACT25950.011.799942
TACTCGA63900.011.4843465
GTAGAAC31050.011.158030511
AGTACTC70150.011.0399733
GCGTGAC34300.09.9781571
CTCTATA71150.09.29552845
GGAAAAT107550.09.1280281
AATCGAA42300.08.9404315
CACTCGT73950.08.85261535
GAATCGA32350.08.8374584
GCGGAAA79850.08.5723431
ACTCGTG76150.08.53765936
GTGACTC42450.08.3794733
AAAATCC75950.08.2701553
TAGAACA43350.08.19938612
TCACTCG78800.08.13661434
GGTAGAA42900.08.12834310
GGGAAAT54000.08.04756451
CACGTAA42450.08.00218316
Produced by FastQC (version 0.11.5)