FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n02_E2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n02_E2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25675051
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27100.013.1273832
GTACTCG59000.011.7896574
GCGTGAC35300.011.3542081
TACTCGA63000.010.9335925
AGTACTC68300.010.6139353
CTCTATA59100.09.89654245
CACTCGT59950.09.64408435
GCGGAAA66200.09.5918571
ACTCGTG60900.09.5304936
CTCGAAT22300.09.1885741
GTAGAAC27000.09.1655711
GTGACTC42100.09.1444023
GGAAAAT107800.09.1279721
TCACTCG62050.09.10031834
GAATTGA54850.08.3313264
AAAATCC80300.08.2988413
AATCGAA44000.08.2367375
CGGAAAC76950.08.2222012
CACGTAA44800.08.08449816
GCTAGTT77350.08.05615912
Produced by FastQC (version 0.11.5)