FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n02_E2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n02_E2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24523154
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG59000.011.4845854
CGTGACT21600.010.8404612
TACTCGA63300.010.7041465
AGTACTC68350.010.6388573
GTAGAAC31750.09.77835711
GAATCGA34600.08.97854
GGAAAAT110500.08.7212361
AAAATCC80800.08.5537783
AATCGAA42500.08.4216565
GCGTGAC30950.08.1480551
GCATAGG34100.07.91671341
TCAGGTA45950.07.88238337
GGTAGAA42850.07.77034810
TAGGCGG35050.07.63994931
TCACACG25400.07.528379415
ACTCGAG100450.07.5255076
AAAAATC61950.07.5231062
AAATCCT85850.07.42073544
TAGAACA43750.07.404539612
TATTCGC39850.07.39537437
Produced by FastQC (version 0.11.5)