FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17759218
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG10500.030.85611715
CGTCTGA10550.030.28893716
CACGTCT12100.026.77438514
CACACGT12850.025.21288712
ACACGTC14100.022.97796213
CAGTCAC9450.021.9417727
TCGGAAG19350.021.3677353
ATCGGAA19750.020.9350322
AGCACAC16850.020.15750710
CGGAAGA21700.019.5702844
GCACACG17200.019.2260911
CCAGTCA11800.019.09307326
GATCGGA22950.017.8459451
ACCGATC5950.016.77980834
AACTCCA19300.016.38972322
GAGACCG6550.016.22729931
CCGAGAC6400.015.86732229
CTCCAGT18100.015.50451324
GAACTCC25200.013.6011321
CTGAACT25400.013.02309119
Produced by FastQC (version 0.11.5)