FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n01_E2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n01_E2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22311262
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC267180.11975118216082982TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46350.027.35157443
CTCGTAT47000.026.73357642
AGTCACG54250.025.75058228
ACGTCTG59700.023.77662515
CGTCTGA60400.023.6872916
CGTATGC53550.023.12755844
GTCACGA64800.021.73174329
TCGGAAG75300.019.7823523
CACGTCT74600.019.51015314
ATCGGAA77450.018.9719312
CAGTCAC75200.018.8160327
CACACGT82800.017.74099712
ACACGTC88900.016.77671613
GTATGCC77450.016.3977545
TCTCGTA79400.016.33399841
ACGATCA88450.016.22660832
GATCGGA96500.015.39112951
TCAGATC92250.015.02446736
AGCACAC102600.014.66810310
TCACGAT95900.014.63730830
Produced by FastQC (version 0.11.5)