FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n01_E2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n01_E2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23505833
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG80300.024.73667115
CGTCTGA80400.024.64989316
TCGTATG46650.024.03803443
CTCGTAT48300.023.543242
GTCACAC85500.022.91644529
TCGGAAG97650.020.7614483
ATCGGAA99400.020.2602372
CGTATGC51500.020.1566244
CAGTCAC98450.020.08483927
CACGTCT102750.019.70410714
AGTCACA102600.019.0751128
CACACGT106300.019.02490612
CACACTT103200.018.96420931
AATCTCG77600.018.7669539
TCACACT111100.017.71695930
CCAGTCA113150.017.3562126
ACACGTC117900.017.34387813
TTGAATC107950.017.3203536
GAATCTC106800.017.00420838
ACTTGAA117000.016.94019934
Produced by FastQC (version 0.11.5)