FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n01_E2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n01_E2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12437343
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT18700.017.09873242
ACGTCTG24100.017.08159815
TCGTATG19700.015.65939643
CGTCTGA26600.015.39161516
TATAGGC26600.014.123075527
CGCGTAT5100.013.2372921
CACGTCT32600.013.17984214
CGTATGC24650.012.51491344
TTATAGG31700.011.99283626
CAGTCAC34300.011.73960127
TCGGAAG36450.011.6057113
ATCGGAA35500.011.5993632
CTGGATT36400.011.3753441
ACTATTA33300.011.34955119
CACACGT39250.011.2333912
AGTCACC37150.011.20230428
ACACGTC40450.010.90013813
ATACTAT35800.010.87091917
TACTATT35750.010.76053118
CATACTA35600.010.42625616
Produced by FastQC (version 0.11.5)