FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n01_E2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n01_E2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23978070
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC258570.10783603517714312TruSeq Adapter, Index 6 (100% over 51bp)
CAAAAACAAAAGCAGAGCACGGCCGGTAAAATCAAAAGACGATGAACAAAC245670.10245611927899119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG55400.026.88062728
ACGTCTG57450.026.74377615
CTCGTAT52050.026.26072342
TCGTATG52000.026.06956343
CGTCTGA59450.025.99537316
GTCACGC64800.023.18956629
CGTATGC61000.021.66958844
TATCTCG66900.021.63382339
ATCGGAA73250.021.1952952
TCGGAAG74350.021.0329863
CACGTCT77150.020.3522114
CGCCAAT74550.019.85572233
CACACGT81500.019.45913712
ACGCCAA76250.019.44250132
CAGTCAC78350.019.40880827
CACGCCA81100.018.52877831
ACACGTC90250.017.79684813
GCCAATA86500.017.29592534
TCACGCC90000.017.27136630
TCTCGTA84300.016.61435341
Produced by FastQC (version 0.11.5)