FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n01_E2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n01_E2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24152919
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC368860.1527186010105031TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG63400.030.15992515
CGTCTGA63850.029.87690216
CTCGTAT63900.028.08518242
TCGTATG64750.028.06425543
TCGGAAG76900.025.2807273
CGTATGC74900.024.50181444
CACGTCT79700.024.3586414
ATCGGAA79650.024.2105882
GTCACAC79700.023.51187529
CACACGT88800.022.14109412
CAGTCAC85750.022.0366627
AGTCACA90200.020.84972428
ACACGTC98400.020.1638813
AGTGATC91650.019.95833436
CACAGTG95900.019.3528533
TCTCGTA96350.018.88219341
GATCGGA105050.018.5303781
GTATGCC100200.018.47253845
ACACAGT101900.018.25748432
CGGAAGA108900.018.079334
Produced by FastQC (version 0.11.5)