FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n01_E2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n01_E2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27000950
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG62400.023.10847315
CTCGTAT49050.023.09104542
TCGTATG48650.022.58676143
CGTCTGA64750.021.99184816
AGTCACT70550.019.89689828
CGTATGC55600.019.1155844
ACTGACC77750.018.25726132
TCGGAAG81750.018.0563743
ATCGGAA82000.017.8916592
CACGTCT82950.017.57342514
CAGTCAC86550.016.53057127
AATCTCG79400.016.29989839
CACACGT94150.015.55458712
CACTGAC93350.015.25412331
ACACGTC100950.014.81880413
GTCACTG98400.014.28837229
AGCACAC115900.013.31491810
CTGACCA109400.013.28378633
CCAGTCA108500.013.26930126
TGACCAA107800.013.21029534
Produced by FastQC (version 0.11.5)