FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n01_E2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n01_E2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25972064
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC268970.10356127260428745TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG50050.025.2848943
CTCGTAT53350.024.22732542
ACTTAGG62950.024.15747332
ACGTCTG65750.023.57308815
CGTCTGA69700.022.23716716
TTAGGCA68450.021.75768334
TCACTTA69550.021.605930
CGTATGC59000.021.22038744
GTCACTT74650.020.0695429
AGTCACT74500.020.01936328
CACGTCT79100.019.73676514
ATCGGAA82850.018.957332
TCGGAAG83250.018.865993
CTTAGGC83700.018.49116933
CACACGT90150.017.49223512
CAGTCAC87000.017.29815927
ACACGTC101650.015.69032313
AGCACAC112550.014.33067510
CACTTAG106800.014.21757631
GTATGCC93900.014.10075245
Produced by FastQC (version 0.11.5)