FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n01_E2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n01_E2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25675051
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC414320.1613706629054018TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG65650.029.36115643
CTCGTAT67200.029.18639642
CGTCTGA83900.027.77741816
ACGTCTG84150.027.64148315
CGTATGC73200.026.02531444
GATGTAT86500.025.67278535
CACCGAT91400.024.48899731
ACCGATG94250.023.77313632
TATCTCG90250.023.66843239
ATCGGAA101650.023.3313942
CACGTCT100450.023.26808214
TCGGAAG102550.023.1702463
CAGTCAC102400.022.20983527
CACACGT111850.021.27867712
AGTCACC109600.020.79184328
GTATCTC109550.020.27399338
TGTATCT112200.019.97394237
ACACGTC122150.019.68698513
TCTCGTA101600.019.59159741
CCAGTCA124550.018.368426
Produced by FastQC (version 0.11.5)