FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l05_n01_E2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l05_n01_E2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24523154
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC807380.32923171301701243TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG130550.035.7036515
TCGTATG115300.035.48496643
CTCGTAT110400.035.4485642
CGTCTGA131950.035.23944516
CGTATGC120950.033.25034744
ATCGGAA149000.031.6986712
CACGTCT148450.031.58037214
TCGGAAG150200.031.4751913
CACACGT153650.030.62872112
GTCACAT151850.029.98428329
CAGTCAC154300.029.49361227
AGTCACA155450.029.10176528
ACACGTC165250.028.56036613
TCACGAT161900.027.48888435
GATCGGA173800.027.3198661
ATCACGA166650.027.13459234
AGCACAC177350.026.8781610
TCTCGTA147000.026.72845641
CCAGTCA173000.026.38359326
GTATGCC153650.026.36447745
Produced by FastQC (version 0.11.5)