FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16347215
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT10500.024.6583942
GTAGATC7050.022.97807932
GAGTGTA7150.022.97574428
TAGATCT7300.022.19007333
GTGTAGA7600.021.91170730
AGTGTAG7850.020.92739929
TCTCGGT7800.020.76553537
TCCCTAC6350.017.714799
TTCCCTA6850.017.407428
TGTAGAT9700.017.16557131
GATCTCG10100.016.48165935
GTGTAGG10150.016.40097217
TGTAGGG10350.016.08399418
GTAGGGA10300.015.943665519
TCGTGTA10450.015.92969115
TAGGGAA10500.015.8556320
TACACGA5250.015.85456313
CCCTACA7150.014.78803110
TCGGTGG11750.014.55104539
CTCGGTG11800.014.29909138
Produced by FastQC (version 0.11.5)