FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n02_D2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n02_D2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19771819
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG45100.013.0286444
GTAGAAC23200.012.99358211
CGTGACT21800.012.6001262
TACTCGA45850.012.5206315
AGTACTC50500.011.9481813
GCGTGAC28700.010.1984221
TAGAACA32950.09.55824312
TCAGGTA35450.09.329787
GGAAAAT87050.09.1301411
AAAATCC64350.09.0616983
TATAAGG24100.09.0610825
GGTAGAA34900.08.95995510
CTCTATA36850.08.79035345
CGAGCAA103700.08.6843963
TCTATCC31350.08.6839677
CGGCTAT25150.08.683711
CACTCGT39000.08.53660135
AAATCCT69200.08.4912124
TCACACG22900.08.44791915
GAATTGA45050.08.3455884
Produced by FastQC (version 0.11.5)