FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n02_D2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n02_D2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23651175
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG52500.012.3929524
CGTGACT21000.011.7935752
TACTCGA53700.011.6126075
GTAGAAC29100.011.51903411
AGTACTC60200.011.2946883
CACTCGT46100.010.00324335
GCGGAAA53350.09.7909891
ACTCGTG48350.09.67745536
GCGTGAC29750.09.6114681
CTCTATA50500.09.26509245
TCACTCG51050.09.03405634
AAAATCC70150.08.954483
GGAAAAT97000.08.7506961
GGTAGAA38850.08.74397210
TCAGGTA41850.08.6556587
GTGACTC33900.08.3018353
TAGAACA42300.08.19015612
TATAAGG25650.08.16243655
AATCGAA46350.08.1598815
TCGTGAG59850.08.11937338
Produced by FastQC (version 0.11.5)