FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n02_D2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n02_D2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27278044
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC45000.011.59807711
CGTGACT22700.010.2163652
TCAGGTA53600.010.0742867
GTACTCG48500.09.5160624
GGTAGAA58300.08.99087210
CAGGTAG61300.08.7348868
AAAAATC85700.08.7225272
TAGAACA60850.08.57697112
GGAAAAT105050.08.4876171
TACTCGA53200.08.4211515
CACTCGT49450.08.324835
CTCGAAT25300.08.187551
AGTACTC61050.08.1135193
GAATCGA45250.08.1098754
AAAATCC78600.08.1065523
GCGGAAA57400.08.0805691
GAATTGA68350.08.0370464
AGATCAG106200.07.9073234
AATTGAC46600.07.8263475
CTAAGCG24650.07.7578758
Produced by FastQC (version 0.11.5)