FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n02_D2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n02_D2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28479944
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC39450.010.32154511
CACTCGT57750.09.11505835
GGAAAAT108000.08.8187611
CGCGATA12800.08.79079329
CTCTATA61100.08.65171945
GTACTCG40850.08.5424664
ACTCGTG61550.08.47928636
TCAGGTA55050.08.4601047
GCGGAAA64000.08.4434941
GGTAGAA49650.08.42768710
CGTGACT22250.08.3992212
TCACTCG62500.08.24300134
TACTCGA42000.07.9331175
AAAATCC79300.07.7511593
CAGGTAG57000.07.6966128
CGGAAAC73500.07.50532772
TAGAACA56100.07.498656312
AGTACTC50750.07.49769643
CTCGAAT25050.07.3705021
AATCGAA51750.07.3520385
Produced by FastQC (version 0.11.5)