FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n02_D2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n02_D2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16939696
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC23500.012.92359511
CGTGACT13450.012.7226342
GTACTCG32900.012.5227554
TACTCGA34900.011.6756175
AGTACTC43200.010.4237263
TCAGGTA32000.09.983947
AAAATCC50200.09.6878373
CACTCGT30200.09.45983235
GGTAGAA32700.09.42522110
GGAAAAT70000.09.3601321
CTCTATA31600.09.11164345
TAGAACA33150.09.02563812
GCGGAAA36150.08.9066571
CAGGTAG35950.08.824068
ACTCGTG32850.08.69683836
GCGTGAC21800.08.5725231
TCACTCG33300.08.24217834
TATAAGG17900.08.1749865
GTGACTC22900.07.96390873
AATCGAA31400.07.9582955
Produced by FastQC (version 0.11.5)