FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n02_D2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n02_D2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25627655
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC29100.011.98268611
GTACTCG51100.011.6753174
TACTCGA53650.010.868225
CGTGACT23000.010.3766562
AGTACTC64000.09.8501533
GCGGAAA58800.09.2282621
CACTCGT51500.09.172749535
CTCTATA54300.09.072494545
ACTCGTG54500.08.75042936
CTCGAAT22950.08.7314731
CGCGATA11000.08.59240129
GGTAGAA42500.08.5751810
TCAGGTA48250.08.4867647
TAGAACA41900.08.3756812
TCACTCG56900.08.30285734
GGAAAAT101500.08.251961
AATTGAC39400.08.2280085
GAATCGA40800.08.1114944
CGATAGT12450.07.952194731
CGGAAAC70250.07.94849062
Produced by FastQC (version 0.11.5)