FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n02_D2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n02_D2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22381982
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT20750.012.6951272
GTACTCG47200.012.0672954
GTAGAAC29200.011.71058111
TACTCGA47850.011.6678665
AGTACTC56450.010.9679243
GCGTGAC28850.09.8332031
GGAAAAT98800.09.4799621
AAAATCC73500.09.0362943
TCAGGTA41850.09.031947
TAGAACA38850.08.80161812
GGTAGAA41900.08.64431410
GTGACTC33000.08.4598663
GAATTGA51850.08.4233414
AATCGAA40350.08.3131275
GCGGAAA48250.08.0260411
GAATCGA34350.07.9303074
CGAGCAA107950.07.8836053
ACTTTCG12500.07.738533519
CTCGAAT18650.07.72629741
CTAGCAC39650.07.715859414
Produced by FastQC (version 0.11.5)