FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n02_D2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n02_D2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24253648
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23400.013.7594372
GTACTCG67100.012.5482964
TACTCGA67350.012.2339485
AGTACTC74750.011.6563673
GTAGAAC31900.011.21293911
GCGTGAC33250.010.5636591
GCGGAAA60050.09.9360611
AAAATCC79850.09.5866633
GGAAAAT106500.09.386741
GTGACTC37300.09.1748343
CTCTATA57400.09.13111745
CACTCGT58150.08.97475535
ACTCGTG58350.08.86707336
GGTAGAA42600.08.71339510
AAATCCT85850.08.6014014
ACTCGAG105400.08.5813016
TCAGGTA45300.08.5427557
TCACTCG61000.08.48249434
CGGAAAC68300.08.439172
GACGTAA8300.08.40502625
Produced by FastQC (version 0.11.5)