FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n02_D2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n02_D2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23877863
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA62500.012.2465595
GTACTCG63800.012.1386724
CGTGACT22150.011.9942622
AGTACTC66750.011.9399563
GTAGAAC28050.09.94499411
GCGTGAC31650.09.31891
GGAAAAT108450.09.1138531
AATTGAC38450.08.8408865
AAAATCC77900.08.6703073
CTCGAAT20000.08.6681791
AATCGAA47750.08.5333655
CCCGAAT16100.08.5304331
ACTCGAG99000.08.4769636
TCACACG24350.08.40678415
TTACGAC24600.08.2332729
GAATTGA58200.07.8524734
CGAAAAT22950.07.84827761
GTGACTC36500.07.8336033
CACTCGT46500.07.691865435
GAATCGA38950.07.68736654
Produced by FastQC (version 0.11.5)