FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16347215
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG7900.027.16806615
CGTCTGA8500.025.51389116
AGACCGA6850.022.78651232
CACACGT9700.022.35493312
CAGTCAC7800.022.26363627
CACGTCT10300.021.4889514
GAGACCG7350.020.8917131
CCGAGAC7750.020.0990329
ACCGATC7700.019.74104534
ACACGTC11100.019.33387813
CCAGTCA10550.017.74162726
AGCGGTT9450.017.0323311
AGCACAC13150.016.8299510
GGAATGC9550.016.42364323
TCGGAAG17650.015.8353523
CGGTTCA10200.015.77985713
ATCGGAA18500.015.1130522
CCGATCT10400.015.05745235
CTCCAGT14500.014.23227224
GCACACG15300.014.17273611
Produced by FastQC (version 0.11.5)