FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n01_D2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n01_D2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19771819
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAACAAAAGCAGAGCACGGCCGGTAAAATCAAAAGACGATGAACAAAC201420.10187226577382687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG39250.026.13445528
ACGTCTG41800.025.34737215
CGTCTGA42450.024.90625816
CTCGTAT38850.023.99863842
TCGTATG39350.023.92274743
CGTATGC44050.021.11485144
TCGGAAG55100.020.0496163
GTCACGA52900.019.73106829
CACGTCT56150.019.3902714
ATCGGAA56250.019.3198642
CACACGT58650.018.67881212
CAGTCAC61450.017.24198727
TCACGAT62150.016.64964530
ACACGTC72150.015.30852913
TCTCGTA63100.014.88266641
ACGATCA73450.014.67006432
GATCGGA75850.014.566451
AGCACAC78600.014.13815510
CCAGTCA75800.014.03719226
GAGCACA83700.013.7067039
Produced by FastQC (version 0.11.5)