FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n01_D2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n01_D2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23651175
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG66400.021.71619215
CTCGTAT40500.021.29589542
CGTCTGA70200.020.66885216
TCGTATG38150.020.30560943
GTCACAC76050.018.90146329
TCGGAAG84150.017.7276233
ATCGGAA83900.017.619762
CGTATGC44000.017.29895244
CAGTCAC84050.017.15592227
CACGTCT85950.016.95988314
CACACGT87450.016.84904312
AGTCACA86550.016.58239728
AATCTCG68750.015.78286639
CACACTT92900.015.73951631
CCAGTCA96950.014.9195926
ACACGTC101350.014.8045713
TCACACT100650.014.57228130
GAATCTC94150.014.41329938
TCCAGTC104650.014.03678525
TTGAATC103100.013.72566736
Produced by FastQC (version 0.11.5)