FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n01_D2S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n01_D2S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27278044
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT46550.020.41445242
ACGTCTG62150.019.0750315
TCGTATG47700.018.97816343
CGTCTGA66000.017.86006516
CGTATGC55150.015.883924544
ATCGGAA79600.015.3489272
CACGTCT80150.015.24024814
TCGGAAG81600.015.1103493
CACACGT82700.014.71592212
CAGTCAC86450.013.92145227
ACACGTC93250.013.38874413
AGTCACC95100.013.10463728
TATAGGC53950.012.13381227
TCTCGTA83400.011.93423941
GATCGGA105500.011.7737211
CCAGTCA102950.011.55912826
CGGAAGA110200.011.4337984
TCCAGTC106550.011.33748125
GTATGCC82700.011.19154445
TTATAGG63750.010.23324926
Produced by FastQC (version 0.11.5)