FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n01_D2S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n01_D2S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28479944
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC563140.19773213037216647TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG104100.030.36097528
CTCGTAT94850.029.654742
TCGTATG94300.029.25471543
ACGTCTG114050.028.58006515
CGTCTGA117150.027.90059316
CGTATGC97850.027.84832644
GTCACGC115900.027.4057329
TATCTCG111100.026.97111339
CGCCAAT123850.025.15639733
ATCGGAA133300.024.9139392
TCGGAAG135250.024.537713
CACGTCT133250.024.47884414
ACGCCAA127800.024.29060632
CAGTCAC133800.024.0587827
CACGCCA133100.023.81348831
CACACGT140050.023.46697812
GTATGCC118650.023.27004845
TCACGCC140150.022.92055930
ACACGTC148650.022.47251313
GCCAATA140200.022.19105734
Produced by FastQC (version 0.11.5)