FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n01_D2S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n01_D2S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16939696
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG30750.021.67765443
ACGTCTG32400.021.52337515
CTCGTAT30500.021.48603442
CGTCTGA33300.021.14432316
CGTATGC33500.019.62948244
ATCGGAA41850.016.7207132
TCGGAAG41500.016.698883
GTCACAC42350.016.25400529
CACGTCT44350.016.0790114
CACACGT48950.014.61396712
CAGTCAC52150.014.01914227
ACACGTC51500.013.84668313
AGTCACA51150.013.58955528
AGTGATC51800.013.15967136
CGCGTAT8050.013.1381471
GTATGCC52700.013.11893545
CACAGTG52250.012.91613333
ACACAGT53800.012.83655532
TCTCGTA55800.012.30887941
GATCGGA60350.011.6334751
Produced by FastQC (version 0.11.5)