FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n01_D2S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n01_D2S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25627655
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG40900.018.53528815
CGTCTGA46500.016.25470516
TCGTATG35500.015.79522343
CTCGTAT37050.015.6206442
AGTCACT51050.014.58562328
CACGTCT59100.013.62662514
TCGGAAG58650.013.2736593
CGTATGC40700.013.22409344
ATCGGAA58300.013.199232
ACTGACC60350.012.33796332
CACACGT67050.011.87673912
CGCTATG15600.011.82447929
AATCTCG61000.011.36631439
CAGTCAC67550.011.256000527
ACACGTC75900.010.78828313
TATAGGC47250.010.56924327
GTCACTG74650.010.39637829
CACTGAC76850.010.04021531
CCCGACT18700.09.7468661
ATCGGTG82500.09.4889476
Produced by FastQC (version 0.11.5)