FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n01_D2S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n01_D2S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22381982
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC225740.10085791329829503TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG49500.027.08528732
CTCGTAT44400.025.91757242
TCGTATG43800.025.24449743
ACGTCTG55200.025.02198215
CGTCTGA58450.023.82311416
TTAGGCA58700.022.87901934
TCACTTA60150.022.62622830
CTTAGGC59600.022.60876733
CGTATGC49450.022.26920944
AGTCACT61750.022.0399628
GTCACTT64300.021.20088829
CACTTAG64650.020.8773431
ATCGGAA68450.020.4457242
TCGGAAG71250.019.9579013
CACGTCT73500.019.0980814
CAGTCAC75850.018.38774527
CACACGT77850.018.20431312
TAGGCAT80400.016.9571835
ACACGTC86350.016.542613
TCTCGTA71250.016.46665441
Produced by FastQC (version 0.11.5)