FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n01_D2S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n01_D2S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24253648
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC309740.1277086234615098TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT55800.026.79755242
TCGTATG54900.026.33464843
ACGTCTG68600.025.57785615
CGTCTGA70900.024.71637716
CGTATGC59950.023.55288544
GATGTAT76050.022.18714135
ACCGATG78900.021.41192232
TATCTCG75100.021.37296539
CACCGAT78650.021.33697331
ATCGGAA85250.020.824382
TCGGAAG86950.020.6497043
CACGTCT86350.020.50246214
CACACGT89900.019.8429912
CAGTCAC89400.019.4758427
GTATCTC91850.018.6452238
TGTATCT93450.018.32213237
TCTCGTA85800.017.79501241
AGTCACC100500.017.75006128
ACACGTC106650.017.0007413
AGCACAC112750.016.12083610
Produced by FastQC (version 0.11.5)