FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l04_n01_D2S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l04_n01_D2S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23877863
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC399700.16739353936321688TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT65200.029.39329342
TCGTATG68800.029.26276843
ACGTCTG82250.027.65095915
CGTATGC74200.026.86037344
CGTCTGA85300.026.5567816
ATCGGAA98400.023.689162
TCGGAAG98850.023.5580143
CACGTCT99000.023.29075814
CACACGT101550.022.77236612
CAGTCAC99150.022.64289327
GTCACAT99050.022.52948229
AGTCACA103050.021.78595528
TCACGAT102650.021.5664835
TCTCGTA96250.020.49572641
ATCACGA110550.020.28814134
ACACGTC117050.019.7952413
GTATGCC105650.019.37613145
CATCACG115750.019.35690933
GATCGGA123250.019.060251
CCAGTCA121750.018.66149526
Produced by FastQC (version 0.11.5)