FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17746396
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT10750.025.5457632
GTAGATC6900.023.48157532
TCTCGGT7150.023.28100637
AGTGTAG6950.022.66960729
GAGTGTA7200.022.50653828
GTGTAGA7450.022.05313930
TAGATCT7600.021.90913433
TGTAGAT8600.018.84236531
TTCCCTA7400.018.5464128
TCCCTAC6900.017.2816859
GTGTAGG9750.016.84411417
TGTAGGG9700.016.6984918
GATCTCG10600.016.55287235
TACACGA6600.016.3615613
CTCGGTG11100.015.80729238
TAGGGAA10250.015.80260820
CCCTACA7600.015.68928710
TCGTGTA10400.015.57486115
GTAGGGA10400.015.35806719
TCGGTGG10900.014.85932939
Produced by FastQC (version 0.11.5)