FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n02_A2S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n02_A2S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18788117
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG40850.011.1851194
CGTGACT16300.010.6336832
TACTCGA42150.010.6264055
AGTACTC45600.010.4652153
GTAGAAC20700.09.67252711
CTCTATA34900.09.28134945
CGGCTAT27000.09.1714841
TCACTCG34750.09.12797134
CACTCGT33700.09.07785135
AATCGAA37450.08.7145915
GCGGAAA39100.08.636251
ACTCGTG36750.08.6305236
GGAAAAT73100.08.5920641
CTCGAAT18250.08.511321
TCCGTTA19100.08.2446319
ACTTTCG12300.08.04737119
GCGTGAC25350.07.90355971
AAAATCC57050.07.8913533
GCGAATT27650.07.89104442
CGCGAAT28150.07.830664641
Produced by FastQC (version 0.11.5)