FastQCFastQC Report
Wed 24 Apr 2019
CCRMBACXX_l03_n02_A2S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCRMBACXX_l03_n02_A2S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20092558
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAATCC72900.08.8619153
CGTGACT16050.08.5552852
GGAAAAT89400.08.5362431
AAAAATC73950.08.31006052
GTAGAAC27000.08.24889811
AAATCCT72050.08.1847184
GAATCGA38100.07.85711534
AATCCTA38900.07.8111155
GGGGAAT44400.07.65594151
GTACTCG35250.07.5345854
CACTCGT27800.07.444491435
TCACTCG27600.07.417486734
AAATCCA82550.07.3345194
AATCGAA48550.07.3248165
CGTTAAC15100.07.30027621
TACTCGA35400.07.2482055
GCGGAAA37000.07.2401881
CGCGATA8155.638867E-117.18074529
TCAGGTA39400.07.13785037
AATTGAC32450.07.07480345
Produced by FastQC (version 0.11.5)